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67060 Sigma

Methyl Green

zinc chloride salt, for microscopy (Bact., Bot., Hist.)

Synonym: Ethyl Green

  • CAS Number 7114-03-6

  • Linear Formula C27H35BrClN3 · ZnCl2

  • Molecular Weight 653.24

  •  Beilstein Registry Number 3914106

  •  Colour Index Number 42590

  •  EC Number 230-415-4

  •  MDL number MFCD00151094

  •  PubChem Substance ID 329760277

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Properties

Related Categories Cell Biology, Hematology and Histology, M, Stains & Dyes, A to Z, Stains and Dyes More...
grade   for microscopy (Bact., Bot., Hist.)
InChI Key   RRKSQWOQZFIQKW-UHFFFAOYSA-J
pH range   0.2 - 1.8, yellow to blue
mp   >300 °C(lit.)

Description

Analysis Note

Material may be a mixture of monobromo-monochloro and dichloro ZnCl2 salts.

Application

Methyl green has been used in the histological staining of ovaries. It has also been used as a counterstain in various cells.

Biochem/physiol Actions

Marker dye for DNA in acid and neutral media.
Methyl green is mainly used as a DNA stain in histochemistry. At low concentrations it binds selectively to AT-rich regions of native DNA. However, at high concentrations it is present at all available DNA sites.

Price and Availability


Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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