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68268 Sigma

Imidazole buffer Solution

BioUltra, 1 M in H2O

Synonym: Glyoxaline solution, Imidazole solution

  • CAS Number 288-32-4

  • Empirical Formula (Hill Notation) C3H4N2

  • Molecular Weight 68.08

  •  Beilstein Registry Number 103853

  •  MDL number MFCD00005183

  •  PubChem Substance ID 24885729

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Properties

Related Categories Alphabetical, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers, Buffer Solutions,
InChI Key   RAXXELZNTBOGNW-UHFFFAOYSA-N
product line   BioUltra
concentration   1 M in H2O
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
pH   9.5±0.5
density   1.01 g/mL at 20 °C

Description

Application

Imidazole can be used as a buffer in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for assay of horseradish peroxidase and as chelator for the binding of various divalent cations. Imidazole can be used for the elution of histidine containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for detection of proteins.

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

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BioUltra Biological Buffers

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