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70706 Sigma

Atto 520

BioReagent, suitable for fluorescence, ≥90.0% (HPCE/HPLC)

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Properties

Related Categories Atto Labels, Biochemicals and Reagents, Detection, Fluorescent Labels, Fluorescent Probes, Labels, Particles and Stains,
InChI Key   YWLGQYDVSLBBTP-BMGIYVBOSA-N
product line   BioReagent
assay   ≥90.0% (HPCE/HPLC)
fluorescence   λex 520 nm; λem 540 nm in 0.1 M phosphate pH 7.0
suitability   suitable for fluorescence
storage temp.   2-8°C

Description

Application

Atto fluorescent labels are designed for high sensitivity applications, including single-molecule detection. Atto labels have rigid structures that do not show any cis-trans isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.
Atto 520 shows a molar extinction of 110,000 and QY of 90% in water (95% in ethanol). Decay time is 3.6 ns.

General description

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Other Notes

Employed in confocal fluorescence microscopy to confirm formation of polymer nanoshells; using combined imaging and scanning fluorescence correlation spectroscopy to characterize microsecond emission fluctuation.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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