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74385 Sigma

Nonidet P 40 Substitute

BioXtra, mixture of 15 homologues

Synonym: 4-Nonylphenyl-polyethylene glycol, Imbentin-N/52, NP 40



Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Culture, Core Bioreagents, Detergents,
description   non-ionic
product line   BioXtra
mol wt   average mol wt 680
TLC   corresponds to standard
refractive index   n20/D 1.49
pH   5-8 (5% in H2O)
CMC   0.059 mM(20-25°C)
solubility   Do you have solubility information on this product that you would like to share?
transition temp   cloud point 45-50 °C
density   1.06 g/mL at 20 °C
anion traces   chloride (Cl-): ≤500 mg/kg
  sulfate (SO42-): ≤100 mg/kg
cation traces   Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤1000 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Zn: ≤5 mg/kg


Frequently Asked Questions

Frequently Asked Questions are available for this Product.


Non-ionic surfactant, used to solubilize cerebral GABA receptors. Used in solubilization of proteins and lipids of the scallop gill ciliary membrane and of membranes from breast cancer tumors.


May become turbid on storage; a clear product can be obtained on heating to 40°C.

Legal Information

Nonidet is a trademark of Shell Chemical Co.

Price and Availability

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Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
UN 3082 9 / PGIII
WGK Germany 
Flash Point(F) 
235.4 °F
Flash Point(C) 
113 °C

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What is the concentration of Product 74385, Nonidet™ P 40 Substitute?
The concentration is 1.77 M based on the density of 1.06 g/mL and an average molecular weight of 600 grams per mole.
How does Product 74385, Nonidet™ P 40 Substitute, compare to Nonidet™ P 40?
Shell Oil, who owned the trademark for the compound, has ceased manufacturing it.The original Shell product and Nonidet™ P 40 Substitute are both characterized as nonionic detergents. Product number 74385 is a mixture of octyl and nonyl phenol ethylene oxide condensate with an average of 9.5 moles of ethylene oxides per mole of phenol. Nonidet™ P 40 was an octyl phenol ethylene oxide condensate with an average of 9 moles of ethylene oxides per mole of phenol.
What is the molecular weight of Product 74385,  Nonidet™ P 40 Substitute?
The molecular weight of this product is approximately 600 g/mol.
What is Product 74385, Nonidet™ P 40 Substitute, soluble in?
This product is soluble in water, ethanol, isopropanol, ether, acetone, and benzene, according to Data for Biochemical Research, 3rd ed., pp. 288-289 (Oxford, 1986).
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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