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74721 Sigma

Nystatin

≥70% (UV), mixture of A1, A2, A3

Synonym: Fungicidin, Mycostatin

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Properties

Related Categories Antibiotics, Antibiotics A to Z, Antibiotics N-S, Antifungal, Biochemicals and Reagents,
InChI Key   VQOXZBDYSJBXMA-QEKUPDCNSA-N
assay   ≥70% (UV)
form   solid
potency   5841 units/mg (USP, dried basis)
color   faintly yellow to light brown
Mode of action   cell membrane | interferes
antibiotic activity spectrum   fungi
  yeast
shipped in   wet ice
storage temp.   −20°C

Description

Biochem/physiol Actions

Mode of Action: Nystatin acts by binding to ergosterols in fungal cell membranes, increasing permeability of the membrane, and creating nystatin/ergosterol based ion channels. It also functions as a lipid raft-inhibiting reagent and as membrane associated cholesterol.

Antimicrobial spectrum: Neomycin acts against fungi, yeasts and molds.

Caution

Nystatin in tissue culuture media is stable at 37°C for three days. It is also stable in moderately alkaline media. Solutions and aqueous suspensions begin to lose activity soon after preparation, with heat, light, and oxygen accelerating the decomposition. Aqueous suspensions are stable for 10 minutes when heating to 100°C at pH 7.

Preparation Note

Nystatin is soluble at 28°C in methanol (11.2 mg/mL), ethanol (1.2 mg/mL), carbon tetrachloride (1.23 mg/mL), chloroform (0.48 mg/mL), benzene (0.28 mg/mL), and ethylene glycol (8.75 mg/mL). It is also soluble in DMSO at 5 mg/mL and freely soluble in DMF and formamide. It is not recommended to autoclave or sterile filter solutions of Nystatin. Nystatin is also effective as a suspension, stable at 37°C for 3 days. Prepare 50 mg/mL stock suspensions in water and store at -20°C.

Application

Nystatin is a polyene antibiotic. Its uses include preventing cell culture contamination and inducing interleukin (IL)-1, IL-8, and tumor necrosis factor a secretion in TLR2-expressing THP1 cells †. This product is recommended for use in cell culture applications at 50 mg/L.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Light sensitive. Store under inert gas.Air sensitive. Air and light sensitive.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
RF5950000

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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