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75403 Sigma

Crystallization Cryo Kit for Proteins

Synonym: Protein Cryo Crystallography Kit

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Description

Components

The kit contains 50 components with 10 mL each. Most components can be ordered separately as 100 mL packages.

Application

The Crystallography Cryo Kit for Proteins provides a rapid, empirical, and efficient screening method to determine the solubility and optimal starting conditions for the crystallization of biological macromolecules in the presence of a cryoprotectant (primarily glycerol). The screening range and probing ability of the Cryo Kit are comparable to those of the Basic Kit. A cryoprotectant is added to freeze crystals in an amorphous glass at -173°C.

Features and Benefits

• Comprehensive, efficient screening
• Stringent purity standards
• Prefabricated reagents, flow charts, and observation sheets
• Low incidence of undesirable solubility effects

Principle

Based on known or published data from various proteins, the Cryo Kit uses a sparse matrix method and minimal amount of protein sample to explore a broad range of buffers, pH, and precipitants. The Protein Cryo Kit is based on the "salting out" principal: increasing the concentration of a precipitating reagent and cryoprotectant depletes the macromolecules of bound water and leads to their precipitation or crystallization.

Price and Availability


Amplified Detection

Kit component also available separately

Description

Product #

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Imidazole pH 6.5; 0.07 M - Sodium acetate 0.7 M - Glycerol 30% solution SDS 71591
Sodium citrate tribasic pH 5.6; 0.07 M – Ammonium phosphate monobasic 0.7 M – Glycerol 30% (v/v) solution SDS 72871
Magnesium chloride 0.18 M – HEPES-Na pH 7.5; 0.09M – 2-Propanol 27% (w/v) – Glycerol 10% solution SDS 72872
Sodium acetate 0.17 M – TRIS hydrochloride pH 8.5; 0.085 M – Polyethylene glycol 4000 25.5% (w/v) – Glycerol 15% (v/v) solution SDS 73803
Ammonium acetate 0.16M – TRIS hydrochloride pH 8.5; 0.08M – 2-Propanol 24% (v/v) – Glycerol 20% (v/v) solution SDS 74613
Sodium citrate tribasic 0.14M – Sodium cacodylate pH 6.5; 0.07M – 2-Propanol 21% (v/v) – Glycerol 30% (v/v) solution SDS 74614
Magnesium chloride 0.2M – HEPES Na pH 7.5; 0.1M – Polyethylene glycol 400 30% (w/v) solution SDS 76525
Ammonium acetate 0.2M – Sodium citrate tribasic pH 5.6; 0.1M – 2-Methyl-2,4-pentanediol 30% (w/v) solution SDS 76658
HEPES Na pH 7.5; 0.065M – Potassium sodium tartrate 0.52M – Glycerol 35% (v/v) solution SDS 76659
Ammonium acetate 0.17M – Sodium citrate tribasic pH 5.6; 0.085M – Polyethylene glycol 4000 25.5% (w/v) – Glycerol 15% (v/v) solution SDS 77437
Ammonium acetate 0.17M – Sodium acetate pH 4.6; 0.085M – Polyethylene glycol 4000 25.5% (w/v) – Glycerol 15% (v/v) solution SDS 77582
Magnesium acetate 0.2M – Sodium cacodylate pH 6.5; 0.1M – 2-Methyl-2,4-pentanediol 30% (v/v) solution SDS 79974
Sodium citrate tribasic 0.2M – TRIS hydrochloride pH 8.5; 0.1M – Polyethylene glycol 400 30% (w/v) solution SDS 80055
Ammonium sulfate 0.17M – Sodium cacodylate pH 6.5; 0.085M – Polyethylene glycol 8000 25.5% (w/v) – Glycerol 15% (v/v) solution SDS 80074
Lithium sulfate 0.17 M – TRIS hydrochloride pH 8.5; 0.085M – Polyethylen glycol 4000 25.5% (w/v) – Glycerol 15% (v/v) solution SDS 80091
Magnesium acetate 0.16 M – Sodium cacodylate pH 6.5; 0.08M – Polyethylene glycol 8000 16% (w/v) – Glycerol 20% solution SDS 80093
Sodium citrate tribasic 0.14M, HEPES Na pH 7.5; 0.07M, 2-Propanol 14% (v/v), Glycerol 30% solution SDS 80384
Sodium acetate 0.17M – Sodium cacodylate pH 6.5; 0.085M – Polyethylene glycol 8000 25.5% (w/v) – Glycerol 15% (v/v) solution SDS 80387
Calcium chloride 0.19 M – HEPES-Na pH 7.5; 0.095M – Polyethylene glycol 400; 26.6% (w/v) – Glycerol 5% solution SDS 80556
Ammonium sulfate 0.17 M – Polyethylene glycol 8000 25.5% (w/v) – Glycerol 15% solution SDS 80803
HEPES-Na pH 7.5; 0.085M – Polyethylene glycol 400 1.7% (v/v) – Ammonium sulfate 1.7M – Glycerol 15% (v/v) solution SDS 80864
HEPES-Na pH 7.5; 0.075M – Sodium phosphate monobasic 0.6M – Potassium phosphate monobasic 0.6M – Glycerol 25% (v/v) solution SDS 80924
Lithium sulfate 0.8 M – Polyethylene glycol 8000 1.6% (w/v) – Glycerol 20% (v/v) solution SDS 81696
Lithium sulfate 0.4 M – Polyethylene glycol 8000 12% (w/v) – Glycerol 20% (v/v) solution SDS 81713
Sodium formate 3.6 M – Glycerol 10% solution SDS 81892
Sodium acetate pH 4.6; 0.07M – 1.4 M Sodium formate – Glycerol 30% (v/v) solution SDS 81893
Sodium acetate pH 4.6; 0.07M – Polyethylene glycol 4000 5.6% (w/v) – Glycerol 30% solution SDS 82216
HEPES-Na pH 7.5; 0.085M – 2-Propanol 8.5% (v/v) – Polyethylene glycol 4000 17% (w/v) – Glycerol 15% (v/v) solution SDS 82809
Sodium acetate pH 4.6; 0.08 M – Ammonium sulfate 1.6 M – Glycerol 20% (v/v) solution SDS 83196
Zinc acetate 0.16M – Sodium cacodylate pH 6.5; 0.08M – Polyethylene glycol 8000 14.4% (w/v) – Glycerol 20% (v/v) SDS 83198
Potassium phosphate monobasic 0.04M – Polyethylene glycol 8000 16% (w/v) – Glycerol 20% (v/v) solution SDS 83617
Calcium acetate 0.16 M – Sodium cacodylate pH 6.5; 0.08M – Polyethylene glycol 8000 14.4% (w/v) – Glycerol 20% SDS 84461
TRIS hydrochloride pH 8.5; 0.08 M – Ammonium phosphate monobasic 1.6 M – Glycerol 20% (v/v) solution SDS 84796
Ammonium sulfate 0.17 M – Polyethylene glycol 4000 25.5% (w/v) – Glycerol 15% solution SDS 87429
Ammonium sulfate 1.5 M – Glycerol 25% solution SDS 89252
TRIS hydrochloride pH 8.5; 0.065M – Polyethylene glycol 8000 5.2% (w/v) – Glycerol 35% (v/v) solution SDS 89725
HEPES-Na pH 7.5; 0.09M – Sodium citrate tribasic 1.26M – Glycerol 10% solution SDS 89734
Polyethylene glycol 1500 24% (w/v) – Glycerol 20% (v/v) solution SDS 90213
Magnesium formate 0.1M – Glycerol 50% (v/v) solution SDS 91638
Sodium citrate tribasic pH 5.6; 0.095M – 2-Propanol 19% (v/v) – Polyethylene glycol 4000 19% (w/v) – Glycerol 5% solution SDS 91959
Calcium chloride 0.02 M – Sodium acetate 0.1M – 2-Methyl-2,4-pentanediol 30% (v/v) solution SDS 92599
Magnesium chloride 0.16M – TRIS hydrochloride pH 8.5; 0.08M – Polyethylene glycol 4000 24% (w/v) – Glycerol 20% (v/v) solution SDS 93189
Sodium citrate tribasic 0.2M – HEPES-Na pH 7.5; 0.1M – 2-Methyl-2,4-pentanediol 30% (v/v) solution SDS 93949
TRIS hydrochloride pH 8.5; 0.075 M – Ammonium sulfate 1.5 M – Glycerol 25% (v/v) solution SDS 94030
Ammonium phosphate monobasic 0.26 M – Glycerol 35% (v/v) solution SDS 96347
Potassium sodium tartrate 0.26 M – Glycerol 35% (v/v) solution SDS 96864
Calcium chloride 0.14M – Sodium acetate pH 4.6; 0.07M – 2-Propanol 14% (v/v) – Glycerol 30% (v/v) solution SDS 73806
Sodium cacodylate pH 6.5; 0.07M – Sodium acetate 0.98M – Glycerol 30% (v/v) solution SDS 79973
Ammonium sulfate 0.16 M – Sodium acetate pH 4.6; 0.08M – Polyethylene glycol 4000 20% (w/v) – Glycerol 20% (v/v) solution SDS 80053
HEPES Na pH 7.5; 0.075M – Lithium sulfate 1.125M – Glycerol 25% (v/v) solution SDS 80084
Safety & Documentation

Safety Information

Signal word 
Danger
Hazard statements 
RIDADR 
UN 3316 9
Protocols & Articles
Peer-Reviewed Papers
15

References

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