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79385 Sigma

Phosphatase, Alkaline from bovine intestinal mucosa

solution (clear), >10000 U/mL

Synonym: Alkaline Phosphatase from calf intestinal mucosa

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Alkaline Phosphatase, Application Index, Biochemicals and Reagents,
form   solution (clear)
specific activity activity   ≥1000 U/mg protein
mol wt   Mr ~140000
concentration   >10000 U/mL
density   1.12 g/mL at 20 °C
storage temp.   2-8°C

Description

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37°C

Physical form

solution in 3 M NaCl, 1 mM MgCl2, 0.1 mM ZnCl2, 30 mM triethanolamine, pH 7.6

Application

Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

Biochem/physiol Actions

Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.

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Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

β-(1—>4)-Galactosyltransferase Kit

β(1→4) Galactosyltransferase from bovine milk (GalT, EC 2.4.1.22) is one of the most extensively studied mammalian glycosyltransferases with regard to synthesis and substrate specificity.[1-5, 9-15] ...
ChemFiles Volume 3 Article 6
Keywords: Biochemistry, Capabilities, Catalysis, Fucosylation, Glycosylations, Organic synthesis, Solvents

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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