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79390 Sigma

Phosphatase, Alkaline from calf intestinal mucosa

for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)

Synonym: Alkaline Phosphatase from calf intestinal mucosa

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Alkaline Phosphatase, Application Index, Biochemicals and Reagents,
grade   for enzyme immunoassay
form   solution (clear, colorless)
mol wt   Mr ~140000
impurities   0.1 mM ZnCl2
  1 mM MgCl2
  3 M NaCl
  30 mM triethanolamine
pH   ~7.6
storage temp.   2-8°C

Description

Other Notes

Sales restrictions may apply

Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase

Reconstitution

The solution can be used without dialysis (for enzyme immuno-assay).

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 °C

Application

Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

Biochem/physiol Actions

Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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