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81188 Sigma

Poly(ethylene glycol)

BioUltra, 1,000

Synonym: PEG

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Properties

Related Categories Analytical Reagents, Analytical/Chromatography, Embedding Media, Embedding and Mounting Media, Essential Chemicals,
vapor density   >1 (vs air)
vapor pressure   <0.01 mmHg ( 20 °C)
product line   BioUltra
autoignition temp.   581 °F
mol wt   Mr 950-1050
impurities   insoluble matter, passes filter test
pH   5.5-7.0 (25 °C, 50 mg/mL in H2O)
solubility   H2O: soluble50 mg/mL at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤200 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤200 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   50 mg/mL in H2O
UV absorption   λ: 260 nm Amax: 0.06
  λ: 280 nm Amax: 0.03

Description

Other Notes

In protein crystallization; As fusogen to obtain hybridomas for monoclonal antibody production; Significantly enhances the transfer of membrane immunoblotting

Application

Poly(ethylene glycol) has been used in the transformation mix solution of yeast. It has been used to prepare an agar-solute substrate to study the influence of cocoon of Cyclorana australis on liquid water exchange with the substrate.

General description

Poly(ethylene glycol) (PEG) is a non-ionic hydrophilic polymer and is available in different molecular weights. It helps in the purification and crystal growth of proteins and nucleic acids. PEG and dextran together result in aqueous polymer two phase system, which is required for the purification of biological materials. PEG also interacts with cell membrane, thereby allowing cell fusion.

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Amplified Detection

The Future of Bioimaging
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles
Peer-Reviewed Papers
15

References

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