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81253 Sigma

Poly(ethylene glycol)

BioUltra, for molecular biology, 6,000

Synonym: PEG

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Properties

Related Categories BioUltra, Essential Chemicals, Materials Science, Molecular Biology, Molecular Biology Reagents,
grade   for molecular biology
vapor density   >1 (vs air)
vapor pressure   <0.01 mmHg ( 20 °C)
product line   BioUltra
form   solid
autoignition temp.   581 °F
mol wt   Mr 5000-7000
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
pH   5.5-7.0 (25 °C, 50 mg/mL in H2O)
solubility   H2O: soluble50 mg/mL at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤200 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤200 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   50 mg/mL in H2O
UV absorption   λ: 260 nm Amax: 0.15
  λ: 280 nm Amax: 0.10
suitability   suitable for (molecular biology applications)

Description

Other Notes

Preparing phage and plasmid DNA - precipitation of λ phage; Fractionation of DNA fragments

Application

Poly(ethylene glycol) has been used for concentrating HIV (human immunodeficiency virus). It has been used in the solution prepared to study CD (circular dichroism) spectra of nisin.

General description

Poly(ethylene glycol) (PEG) is a non-ionic hydrophilic polymer and is available in different molecular weights. It helps in the purification and crystal growth of proteins and nucleic acids. PEG and dextran together result in aqueous polymer two phase system, which is required for the purification of biological materials. PEG also interacts with cell membrane, thereby allowing cell fusion.

Price and Availability


Amplified Detection

The Future of Bioimaging

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles
Peer-Reviewed Papers
15

References

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