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81255 Sigma

Poly(ethylene glycol)

BioUltra, 6,000

Synonym: PEG

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Properties

Related Categories Essential Chemicals, Materials Science, Molecular Biology, Optimization Reagents, PEG and Oligo Ethylene Glycol,
vapor density   >1 (vs air)
vapor pressure   <0.01 mmHg ( 20 °C)
product line   BioUltra
form   solid
autoignition temp.   581 °F
mol wt   Mr 5000-7000
impurities   insoluble matter, passes filter test
  ≤20 mg/kg peroxides (as H2O2)
pH   5.5-7.0 (25 °C, 50 mg/mL in H2O)
solubility   H2O: 50 mg/mL at 20 °C clear, colorless
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤200 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤200 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   50 mg/mL in H2O
UV absorption   λ: 260 nm Amax: 0.15
  λ: 280 nm Amax: 0.1

Description

Other Notes

Fractional precipitation of proteins; Protoplast fusion; In virus purification; Electroblotting of proteins on agarose gel containing PEG and antibodies

Application

Poly(ethylene glycol) has been used for stress (dehydration) treatment in seedlings of Galegae orientalis. It has been used for the preparation of aqueous two-phase system to study protein diffusion across the interface.

General description

Poly(ethylene glycol) (PEG) is a non-ionic hydrophilic polymer and is available in different molecular weights. It helps in the purification and crystal growth of proteins and nucleic acids. PEG and dextran together result in aqueous polymer two phase system, which is required for the purification of biological materials. PEG also interacts with cell membrane, thereby allowing cell fusion.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles
Peer-Reviewed Papers
15

References

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