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81268 Sigma

Poly(ethylene glycol)

BioUltra, for molecular biology, 8,000

Synonym: PEG



Related Categories BioUltra, Essential Chemicals, Materials Science, Molecular Biology, Molecular Biology Reagents,
grade   for molecular biology
vapor density   >1 (vs air)
vapor pressure   <0.01 mmHg ( 20 °C)
product line   BioUltra
form   powder
autoignition temp.   581 °F
mol wt   Mr 7000-9000
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
pH   5.5-7.0 (25 °C, 50 mg/mL in H2O)
mp   59-64 °C
solubility   H2O: 50 mg/mL at 20 °C clear, colorless
density   1.0845 g/mL at 70 °C
  1.0689 g/mL at 90 °C
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤200 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤200 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   50 mg/mL in H2O
UV absorption   λ: 260 nm Amax: 0.09
  λ: 280 nm Amax: 0.03


Other Notes

Precipitant in the large-scale preparation of λgt10 bacteriophage


Poly(ethylene glycol) has been used as a virioplankton concentrating agent. It has been used in the procedure involving ligation of 5′ mRNAs to RNA oligo linker.

General description

Poly(ethylene glycol) (PEG) is a non-ionic hydrophilic polymer and is available in different molecular weights. It helps in the purification and crystal growth of proteins and nucleic acids. PEG and dextran together result in aqueous polymer two phase system, which is required for the purification of biological materials. PEG also interacts with cell membrane, thereby allowing cell fusion.

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NONH for all modes of transport
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