83200 Sigma

Pyronin Y

for microscopy (Bot., Fl., Hist.)

Synonym: Pyronin G, Pyronin J

  • CAS Number 92-32-0

  • Empirical Formula (Hill Notation) C17H19ClN2O

  • Molecular Weight 302.80

  •  Beilstein Registry Number 3795789

  •  Colour Index Number 45005

  •  EC Number 202-147-8

  •  MDL number MFCD00011725

  •  PubChem Substance ID 57652949



Related Categories Biochemicals and Reagents, Cell Biology, Fluorescent Probes, Labels, Particles and Stains, Fluorescent Stains, Hematology and Histology,
grade   for microscopy (Bot., Fl., Hist.)
mp   250-260 °C(lit.)


Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Pyronin Y is important in histochemistry and is combined with methyl green for staining of RNA. The mixture results in bright red/green staining of nucleic acids.

Price and Availability

Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option:
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
Has Pyronin Y, Product 83200, been use-tested for the demonstration of RNA?
No. There are, however, two other Pyronin products from Sigma-Aldrich which should be suitable.  Product No. P9172 has been used to stain RNA in polyacrylamide gels.  Product No. 213519 was submitted to the Biological Stain Commission for certification.  Testing was performed by the Biological Stain Commission to ensure the Pyronin Y stained RNA in tissue sections.
Is there a prepared solution of Methyl Green and Pyronin Y for the demonstration of plasma cells and RNA in tissue sections?
Yes, product HT70116 is available in pre-made form.  The product is approved for in vitro diagnostic (IVD) use.
What is the emission wavelength of Pyronin Y, Product 83200?
The emission is at 570 nm when dissolved in water.
What is the solubility of Pyronin Y, Product 83200, in water and ethanol?
Solubility is not tested at Sigma Aldrich, but the literature reports solubility up to 9% (i.e., 90 mg/mL) in water and 0.6% (6 mg/mL) in ethanol.
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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