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83264 Sigma

HEPES buffer solution

1 M in H2O

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid

  • CAS Number 7365-45-9

  • Empirical Formula (Hill Notation) C8H18N2O4S

  • Molecular Weight 238.30

  •  Beilstein Registry Number 883043

  •  MDL number MFCD00006158

  •  PubChem Substance ID 24888106

  •  eCl@ss 32129211

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffer Solutions, Buffers A to Z,
concentration   1 M in H2O
  238 g/L HEPES in H2O
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
pH   5.5±0.5
density   1.07 g/mL at 20 °C
Featured Industry   Diagnostic Assay Manufacturing

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Safety & Documentation

Safety Information

Protocols & Articles

Peer-Reviewed Papers

References

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Effects of serum-free storage on morphology, phenotype, and viability of ex vivo cultured human conjunctival epithelium. Eidet JR, Utheim OA, Raeder S, et al. Exp. Eye Res. 94(1), 109-16, (2012)

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Biological pH buffers in IVF: help or hindrance to success. Will MA, Clark NA, and Swain JE J. Assist. Reprod. Genet. 28(8), 711-24, (2011)

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What's in your buffer? Solute altered millisecond motions detected by solution NMR. Wong M, Khirich G, and Loria JP Biochemistry 52(37), 6548-58, (2013)

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Direct biomolecule binding on nonfouling surfaces via newly discovered supramolecular self-assembly of lysozyme under physiological conditions. Yang P Macromol. Biosci. 12(8), 1053-9, (2012)

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Hydrothermal synthesis and properties of controlled α-Fe2O3 nanostructures in HEPES solution. Li H, Lu Z, Li Q, et al. Chem. Asian J. 6(9), 2320-31, (2011)

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[Permeability of pannexin 1 channels to large anions]. Romanov RA, Rogachevskaia OA, Kolesnikova AS, et al. Ross. Fiziol. Zh. Im. I. M. Sechenova 98(12), 1578-86, (2012)

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Magnesium alloys: predicting in vivo corrosion with in vitro immersion testing. Walker J, Shadanbaz S, Kirkland NT, et al. J. Biomed. Mater. Res. B. Appl. Biomater. 100(4), 1134-41, (2012)

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Connexin-based channels in astrocytes: how to study their properties. Giaume C, Orellana JA, Abudara V, et al. Methods Mol. Biol. 814, 283-303, (2012)

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Ratiometric fluorescence sensor based on a pyrene derivative and quantification detection of heparin in aqueous solution and serum. Dai Q, Liu W, Zhuang X, et al. Anal. Chem. 83(17), 6559-64, (2011)

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Pyrophosphate sensing by a fluorescent Zn2+ bound triazole linked imino-thiophenyl conjugate of calix[4]arene in HEPES buffer medium: spectroscopy, microscopy, and cellular studies. Pathak RK, Tabbasum K, Rai A, et al. Anal. Chem. 84(11), 5117-23, (2012)

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Transient acidification and subsequent proinflammatory cytokine stimulation of astrocytes induce distinct activation phenotypes. Renner NA, Sansing HA, Inglis FM, et al. J. Cell Physiol. 228(6), 1284-94, (2013)

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Crystal structure of the Rasputin NTF2-like domain from Drosophila melanogaster. Vognsen T and Kristensen O Biochem. Biophys. Res. Commun. 420(1), 188-92, (2012)

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A Zn2+ specific triazole based calix[4]arene conjugate (L) as a fluorescence sensor for histidine and cysteine in HEPES buffer milieu. Pathak RK, Tabbasum K, Rai A, et al. Analyst 137(17), 4069-75, (2012)

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A fluorescence "turn-on" chemodosimeter for Cu2+ in aqueous solution based on the ion promoted oxidation. Li J, Zeng Y, Hu Q, et al. Dalton Trans. 41(13), 3623-6, (2012)

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Mechanism for modulation of gating of connexin26-containing channels by taurine. Locke D, Kieken F, Tao L, et al. J. Gen. Physiol. 138(3), 321-39, (2011)

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High-resolution structures of Neotermes koshunensis β-glucosidase mutants provide insights into the catalytic mechanism and the synthesis of glucoconjugates. Jeng WY, Wang NC, Lin CT, et al. Acta Crystallogr. D Biol. Crystallogr. 68(Pt 7), 829-38, (2012)

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Highly selective fluorescence turn-on sensing of gold ions by a nanoparticle generation/C-I bond cleavage sequence. Park J, Choi S, Kim TI, et al. Analyst 137(19), 4411-4, (2012)

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Tris buffer improves fluorescence yield of ram spermatozoa when evaluating membrane integrity. Yániz JL, Mateos JA, and Santolaria P Microsc. Res. Tech. 75(4), 520-3, (2012)

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pH-dependent inhibition of native GABA(A) receptors by HEPES. Hugel S, Kadiri N, Rodeau JL, et al. Br. J. Pharmacol. 166(8), 2402-16, (2012)

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Role of bicarbonate as a pH buffer and electron sink in microbial dechlorination of chloroethenes. Delgado AG, Parameswaran P, Fajardo-Williams D, et al. Microb. Cell Fact. 11, 128, (2012)

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Proton feedback mediates the cascade of color-opponent signals onto H3 horizontal cells in goldfish retina. Kamiji NL, Yamamoto K, Hirasawa H, et al. Neurosci. Res. 72(4), 306-15, (2012)

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Phosphate and HEPES buffers potently affect the fibrillation and oligomerization mechanism of Alzheimer's Aβ peptide. Garvey M, Tepper K, Haupt C, et al. Biochem. Biophys. Res. Commun. 409(3), 385-8, (2011)

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Magnetosomes on surface: an imaging study approach. Gojzewski H, Makowski M, Hashim A, et al. Scanning 34(3), 159-69, (2012)

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Prestorage in ovo injection of biological buffers: an approach to improve hatchability in long-term stored eggs. Akhlaghi A, Jafari Ahangari Y, Hashemi SR, et al. Poult. Sci. 92(4), 874-81, (2013)

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Further electrophysiological studies on cellular effect of herbicide, bromoxynil, using characean cells. Shimmen T J. Plant Res. 125(6), 749-54, (2012)

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HOPE-preservation of paraffin-embedded sputum samples--a new way of bioprofiling in COPD. Pedersen F, Marwitz S, Seehase S, et al. Respir. Med. 107(4), 587-95, (2013)

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Reactions of buffers in cyanogen bromide-induced ligations. Vogel H, Gerlach C, and Richert C Nucleosides Nucleotides Nucleic Acids 32(1), 17-27, (2013)

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The tissue is the issue: improved methylome analysis from paraffin-embedded tissues by application of the HOPE technique. Marwitz S, Kolarova J, Reck M, et al. Lab. Invest. 94(8), 927-33, (2014)

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