83702 Sigma

Rhodamine 123

BioReagent, for fluorescence, ≥85% (HPLC)

Synonym: 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester



Related Categories Alphabetical, Biochemicals and Reagents, Fluorescent Indicators and Probes, Fluorescent Probes, Labels, Particles and Stains, Lipophilic and Membrane Probes,
grade   for fluorescence
product line   BioReagent
assay   ≥85% (HPLC)
impurities   ≤10% water
solubility   ethanol: soluble20 mg/mL, clear, red
suitability   in accordance for fluorescence


Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.

Other Notes

Supravital mitochondrial-specific dye

Price and Availability

Laboratory Gloves
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option:
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What are the excitation and emission wavelengths for Rhodamine 123 (Product Nos. 83702 and R8004)?
Using ethanol as the solvent, the excitation maximum is  511 nm and the emission maximum is 534 nm.  The recommended excitation and emission wavelengths in PBS are 488 and 515-575 nm, respectively.
Why would one select Rhodamine 123 (Product Nos. 83702 and R8004) instead of other rhodamine compounds?
Due to is comparatively low toxicity, Rhodamine 123 is the dye of choice for visualizing mitochondria or for cell viability measurements.
What is the solubility of Rhodamine 123, (Product Nos. 83702 and R8004)?
Rhodamine 123 is soluble in ethanol at 20 mg/mL, and has been reported soluble in ether, various fats and lipids, and has limited solubility in water (no concentration given).
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Protocols & Articles


Sensor Dyes

In 1998 R.F. Furchgott, L.J. Ignarro, and F. Murad received the Nobel Prize in Physiology and Medicine for their discoveries concerning nitric oxide as a signaling molecule in the cardiovascular syst...
Monika Baeumle
BioFiles 2011, 6.3, 23.
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