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83832 Sigma

Ribonuclease A from bovine pancreas

powder, white, ~100 U/mg protein

Synonym: Pancreatic Ribonuclease, RNase A, Ribonuclease I, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   powder
mol wt   Mr ~13700
  mol wt ~13,700
color   white
foreign activity   protease ≤0.001 U/mg
shipped in   wet ice
storage temp.   −20°C

Description

Analysis Note

Protein determined by E1%/280.

Features and Benefits

• RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA

Other Notes

Sales restrictions may apply

The return of pancreatic ribonucleases, review; Degradation of deoxyribonucleic acids and ribonucleic acids; Action on double-stranded RNA

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes the RNA at a rate constant k = 1 at 25°C and pH 5.0 (Kunitz-units); M. Kunitz, J. Biol. Chem. 164, 563 (1946)

Application

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

WGK Germany 
3
RTECS 
RF0760000

Documents

Certificate of Analysis

Protocols & Articles

Protocols

Enzymatic Assay of Ribonuclease A (EC 3.1.27.5)

This procedure may be used for determination of Ribonuclease A (RNase A) activity. The Spectrophotometric Stop Rate Determination [Absorbance at 300 nm (A300), Light path = 1 cm] is based on the foll...

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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