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84097 Sigma

Sucrose

BioUltra, for molecular biology, ≥99.5% (HPLC)

Synonym: α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopyranosyl β-D-fructofuranoside, β-D-Fructofuranosyl-α-D-glucopyranoside, D(+)-Saccharose, Sugar

  • CAS Number 57-50-1

  • Empirical Formula (Hill Notation) C12H22O11

  • Molecular Weight 342.30

  •  Beilstein Registry Number 90825

  •  EC Number 200-334-9

  •  MDL number MFCD00006626

  •  PubChem Substance ID 57652987

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Properties

Related Categories Alphabetical, BioUltra, Biochemicals and Reagents, Biostabilization Agents, Carbohydrates,
grade   for molecular biology
InChI Key   CZMRCDWAGMRECN-UGDNZRGBSA-N
product line   BioUltra
assay   ≥99.5% (HPLC)
optical activity   [α]20/D +66.5±1°, c = 26% in H2O
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
ign. residue   ≤0.01% (as SO4)
loss   ≤0.1% loss on drying, 20 °C (HV)
pH   5.0-7.0 (25 °C, 1 M in H2O)
mp   185-187 °C(lit.)
solubility   H2O: 1 M at 20 °C clear, colorless
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   1 M in H2O
UV absorption   λ: 260 nm Amax: 0.11
  λ: 280 nm Amax: 0.08

Description

Other Notes

Isopycnic centrifugation in a discontinuous sucrose density gradient e.g. in the isolation of mRNA from membrane-bound polysomes

Application

Sucrose is a widely used nutitional disaccharide of glucose and fructose. Sucrose is used in molecular biology for the preparation of density gradients.

Price and Availability


Microcon Centrifugal Filters

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
WN6500000
Protocols & Articles
Peer-Reviewed Papers
15

References

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