• USA Home
  • 84101801 - MRC-5 Cell Line human

EMAIL THIS PAGE TO A FRIEND
84101801 Sigma

MRC-5 Cell Line human

From fetal lung fibroblast-like

Purchase

Properties

form   cell suspension
shipped in   dry ice
storage temp.   −196°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Established from normal lung tissue of a 14-week-old male fetus. The cells undergo 60-70 population doublings before senescence. The virus susceptibility of this line to human viruses is similar to that of WI-38 cells.
Subculture: Split at 70-80% confluency, approx. 1:3 to 1:6, seed at 2-4 × 104 viable cells/cm2. Trypsinize using 0.25% solution, with or without EDTA, 37 °C and 5% CO2.
Recommended culture medium: EMEM (EBSS) + 2 mM L-Glutamine + 1% NEAA (non-essential amino acids) + 10% FBS.

Price and Availability

Safety & Documentation

Safety Information

WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?