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85011425 Sigma

L929 Cell Line from mouse

mouse C3H/An connective tisue


Related Categories Cell Culture, ECACC Cell Lines, J-N More...
biological source   Adipose from mouse
growth mode   Adherent
karyotype   Not specified
morphology   Fibroblast
products   Not specified
receptors   Not specified
application(s)   cell culture: suitable
shipped in   dry ice
storage temp.   −196°C


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Virus studies: PRV, VSV (Indiana), HSV

Cell Line Origin

Mouse C3H/An connective tissue

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split semi-confluent cultures seeding at 5x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Cell Line Description

Subclone of parental strain L, established by W R Earle in 1940. One of the first to be established in continuous culture. The L strain was derived from normal subcutaneous areolar and adipose tissue of a 100 day old male C3H/An mouse. Cells are APRT+ and HPRT+.

Price and Availability

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Required for Optimal Growth

With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Fetal Bovine Serum

USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

<SC>L</SC>-Glutamine solution

200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture

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