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85020204 Sigma

IMR-90 Cell Line human

Properties

Related Categories Cell Culture, D-I, ECACC Cell Lines More...
biological source   Lung, foetal from human
description   Human Caucasian fetal lung fibroblast
growth mode   Adherent
karyotype   2n = 46, diploid
morphology   Fibroblast
products   Not specified
receptors   Not specified
application(s)   cell culture: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Application

Virus studies

Cell Line Origin

Human Caucasian foetal lung fibroblast

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 11,13
D16S539: 10,13
D5S818: 12,13
D7S820: 9,12
THO1: 8,9.3
TPOX: 8,9
vWA: 16,19

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Cell Line Description

Derived from lung tissue of a 16 week old female Caucasian foetus. The cells have a virus susceptibility similar to WI-38 (Sigma catalogue no. 90020107) and MRC-5 cells (Sigma catalogue no. 84101801). The cells have a finite lifespan. Available as a growing order only.

Price and Availability

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