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85040C Sigma

Bovine Serum Albumin - Fraction V

lyophilized powder

Synonym: Albumin bovine serum, BSA, Bovine Serum Albumin, Bovine albumin

Properties

description   for research or for further manufacturing use
form   lyophilized powder
mol wt   mol wt ~66 kDa
storage temp.   −20°C

Description

Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn2 method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Application

Bovine serum albumin (BSA) makes up approximately 60% of all proteins in animal serum. It is commonly used in cell culture protocols, particularly when protein supplementation is necessary and the other components of serum are unwanted. In cell culture its main role is as a carrier of small molecules. Because of its negative charge, BSA binds water, salts, fatty acids, vitamins and hormones, then carries these bound components between tissues and cells. The binding capacity also makes BSA an effective scavenger for removing toxic substances, including pyrogens, from the medium.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.1

Price and Availability

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