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85555 Sigma

Spectinomycin dihydrochloride pentahydrate

≥98.0% (AT)

  • CAS Number 22189-32-8

  • Empirical Formula (Hill Notation) C14H24N2O7 · 2HCl · 5H2O

  • Molecular Weight 495.35

  •  Beilstein Registry Number 5684150

  •  EC Number 244-554-3

  •  MDL number MFCD00150886

  •  PubChem Substance ID 57653052

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Properties

Related Categories Aminoglycosides, Antibacterial, Antibiotics, Antibiotics A to Z, Antibiotics N-S,
biological source   from synthetic
InChI Key   DCHJOVNPPSBWHK-UXXUFHFZSA-N
assay   ≥98.0% (AT)
form   solid
color   white
solubility   H2O: 50 mg/mL clear, faintly yellow
Mode of action   protein synthesis | interferes
storage temp.   2-8°C

Description

Caution

Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.

Preparation Note

Stock solutions should be prepared at 10 mg/mL in water and filter sterilized.

Application

Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Store under inert gas.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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