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85578 Sigma

Spermidine trihydrochloride

BioXtra, ≥99.5% (AT)

Synonym: N-(3-Aminopropyl)-1,4-butanediamine trihydrochloride

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Properties

InChI Key   LCNBIHVSOPXFMR-UHFFFAOYSA-N
product line   BioXtra
assay   ≥99.0% (TLC)
  ≥99.5% (AT)
impurities   insoluble matter, passes filter test
pH   3.0-6.0 (25 °C, 1 M in H2O)
mp   257-259 °C(lit.)
solubility   H2O: soluble1 M at 20 °C, clear, colorless
anion traces   sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤10 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   1 M in H2O
UV absorption   λ: 260 nm Amax: 0.2
  λ: 280 nm Amax: 0.1

Description

Caution

Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

Other Notes

Buffer component for chromosome isolation in bivariate flow cytogenetic analysis and sorting.

Application

Spermidine trihydrochloride is used for the following applications:
• DNA extraction and restriction endonuclease digestion
• Nuclear transplantation
• During the chemical analyses of the squid species Todaropsis eblanae
• As one of the agents in intercalating drug.
• Platelet aggregation
It may be used in the synthesis of palladium complexes like [sperH3]2[PdCl4]3, [PdCl2(sperH)]2[PdCl4], and [(PdCl2)3(sper)2] by reacting with K2PdCl4 and PdCl2.

Biochem/physiol Actions

Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T4 polynucleotide kinase activity.

General description

Spermidine trihydrochloride is an aliphatic polyamine. Its crystalline structure has been studied. It has been reported to preserve the nuclei and prevent proteolytic degradation of histones during the macronuclei isolation from Tetrahymena pyriformis.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
EJ7023000
Protocols & Articles
Peer-Reviewed Papers
15

References

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