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85639 Sigma

SSC Buffer

for Northern and Southern blotting, powder blend

Synonym: Saline sodium citrate buffer dry blend

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Properties

grade   for molecular biology
product line   BioReagent
form   powder blend
impurities   DNases, none detected
  RNases, none detected
  phosphatases, none detected
  proteases, none detected
solubility   H2O: soluble0.263 g/mL, clear, colorless
storage temp.   room temp

Description

Components

• Convenient pack sizes
• Multi-purpose use in hybridizations
• Easy-to-use powder makes fast, inexpensive solutions

SSC Buffer powder contains 0.3 M sodium citrate in 3M NaCl.

Application

Suitable for:
• Preparing nucleic acids for hybridization
• Hybridization buffer for Northern and Southern blots
• Washing buffer for Northern and Southern blots

General description

SSC Buffer powder is a standard reagent in Southern and Northern blotting procedures. This powder can be used to make SSC working solutions with concentrations ranging from 0.5x to 20x.

Preparation Note

Dissolve 263.5 g in a final volume of 1L molecular biology grade water. The resulting 20x concentrate is approximately pH 7, and can be further diluted with water, as needed.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles
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