85639 Sigma

SSC Buffer

for Northern and Southern blotting, powder blend

Synonym: Saline sodium citrate buffer dry blend



grade   for molecular biology
product line   BioReagent
form   powder blend
impurities   DNases, none detected
  RNases, none detected
  phosphatases, none detected
  proteases, none detected
solubility   H2O: soluble0.263 g/mL, clear, colorless
storage temp.   room temp



• Convenient pack sizes
• Multi-purpose use in hybridizations
• Easy-to-use powder makes fast, inexpensive solutions

SSC Buffer powder contains 0.3 M sodium citrate in 3M NaCl.


Suitable for:
• Preparing nucleic acids for hybridization
• Hybridization buffer for Northern and Southern blots
• Washing buffer for Northern and Southern blots

General description

SSC Buffer powder is a standard reagent in Southern and Northern blotting procedures. This powder can be used to make SSC working solutions with concentrations ranging from 0.5x to 20x.

Preparation Note

Dissolve 263.5 g in a final volume of 1L molecular biology grade water. The resulting 20x concentrate is approximately pH 7, and can be further diluted with water, as needed.

Price and Availability

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles
Related Products

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