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85878 Sigma

Streptavidin from Streptomyces avidinii

affinity purified, lyophilized from 10 mM potassium phosphate, ≥13 U/mg protein

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Properties

Related Categories Antibodies, Biotin/Avidin, Molecular Biology, Plant Biotechnology, Plant Proteomics,
quality   affinity purified
  lyophilized from 10 mM potassium phosphate
mol wt   Mr ~60000
solubility   H2O: soluble1 mg/mL, clear to hazy, colorless to faintly yellow
storage temp.   −20°C

Description

Analysis Note

Binding capability: Streptavidin binds one molecule of biotin per subunit. Comparison of biotin binding and absorbance measurements indicates that ≤5% binding sites are occupied.

Other Notes

Review: Application of the avidin-biotin technique in microbiology; Protein isolated from the bacterium Streptomyces avidinii, having a high affinity for biotin; Avidin-Biotin technology

Packaging

5, 10 mg in glass bottle

Bottomless glass bottle. Contents are inside inserted fused cone.

The sales quantitites 1 mg and 5 mg correspond to ′mg protein′

Unit Definition

1 U corresponds to the amount of protein which binds 1μg (+)-biotin at pH 7.5

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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