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86010202 Sigma

CACO-2 Cell Line human

colon from human (Caucasian colon adenocarcinoma)



Related Categories A-C, Cell Culture, ECACC Cell Lines More...
biological source   colon from human (Caucasian colon adenocarcinoma)
growth mode   Adherent
karyotype   Hypertetraploid, modal no. 96
morphology   Epithelial
products   Not specified
receptors   Not specified
application(s)   cell culture: suitable
shipped in   dry ice
storage temp.   −196°C


Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cell Line Origin

Human Caucasian colon adenocarcinoma

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,13,14
D16S539: 12,13
D5S818: 12,13
D7S820: 11,12
THO1: 6
TPOX: 9,11
vWA: 16,18

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum FBS / FCS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsinor Trypsin/EDTA; 5% CO2; 37°C. NB: During routine subculture the cells should always be subcultured before they achieve confluence. Cells may show the appearance of circular vacuoles in the cytoplasm. These may increase in frequency as the culture density increases to confluence. To reduce their frequency, media change confluent cultures after 2-3 days if not subcultured. Cells can clump if not separated into a single cell suspension when split.

Cell Line Description

Isolated from a primary colonic tumour in a 72-year-old Caucasian male using the explant culture technique. Forms moderately well differentiated adenocarcinomas consistent with colonic primary grade II, in nude mice.

Price and Availability

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Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


Applications of Conducting Polymer Devices in Life Sciences

Leslie H. Jimison1, Dion Khodagholy1, Thomas Doublet1,2, Christophe Bernard2, George G. Malliaras1, and Róisín M. Owens1 1Department of Bioelectronics, Ecole Nationale Supérieure des Mines CMP-EMSE, ...
Keywords: Bacterial conjugations, Cancer, Cell culture, Diagnostic, Diffusion, Electronics, Gastrointestinal, Immunofluorescence, Organic electronics, Semiconductor, Spectroscopy

Contaminated Cell Lines are Bad for Your Health

For many decades cell lines have been fundamentally important tools in many fields of biomedical research, including cancer research. Over previous decades many billions of dollars have been spent, a...
Edward Burnett and Liz Penn, European Collection of Cell Culture (ECACC®)
Don Finley, Market Segment Manager, Sigma® Life Science
Biofiles, Vol. 8, No. 16
Keywords: Amplification, Cancer, Cell culture, Polymerase chain reaction

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