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86010202 Sigma

CACO-2 Cell Line human

colon from human (Caucasian colon adenocarcinoma)

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Properties

Related Categories A-C, Cell Culture, ECACC Cell Lines More...
biological source   colon from human (Caucasian colon adenocarcinoma)
growth mode   Adherent
karyotype   Hypertetraploid, modal no. 96
morphology   Epithelial
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Application

CACO-2 Cell Line human has been used:
• to study if components of the sigma B regulon in Listeria monocytogenes contribute to cell invasion
• for functional analysis of bacteriocin divercin AS7
• to study the intracellular invasion by Listeria ivanovii
• to study the effect of Maslinic acid, a pentacyclic triterpene, on colon cancer cell lines

Other Notes

NaviCyte Scientific holds the exclusive commercial distribution rights to the CACO-2 cell line deposited by the Memorial Sloan-Kettering Cancer Center. Note: All uses of Catalogue Numbers 86010202 and 09042001, other than for research by a non-commercial or academic entity, require a license and use authorization from NaviCyte Scientific under its exclusive arrangement with Memorial Sloan-Kettering Cancer Center. For information on the licensing terms, please contact NaviCyte Scientific via contact@navicyte-scientific.com or (+1) 973-868-6100.

Cell Line Origin

Human Caucasian colon adenocarcinoma

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,13,14
D16S539: 12,13
D5S818: 12,13
D7S820: 11,12
THO1: 6
TPOX: 9,11
vWA: 16,18

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum FBS / FCS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsinor Trypsin/EDTA; 5% CO2; 37°C. NB: During routine subculture the cells should always be subcultured before they achieve confluence. Cells may show the appearance of circular vacuoles in the cytoplasm. These may increase in frequency as the culture density increases to confluence. To reduce their frequency, media change confluent cultures after 2-3 days if not subcultured. Cells can clump if not separated into a single cell suspension when split.

Cell Line Description

Isolated from a primary colonic tumour in a 72-year-old Caucasian male using the explant culture technique. Forms moderately well differentiated adenocarcinomas consistent with colonic primary grade II, in nude mice.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

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My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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Protocols & Articles

Articles

Applications of Conducting Polymer Devices in Life Sciences

Leslie H. Jimison1, Dion Khodagholy1, Thomas Doublet1,2, Christophe Bernard2, George G. Malliaras1, and Róisín M. Owens1 1Department of Bioelectronics, Ecole Nationale Supérieure des Mines CMP-EMSE, ...
Keywords: Bacterial conjugations, Cancer, Cell culture, Diagnostic, Diffusion, Electronics, Gastrointestinal, Immunofluorescence, Organic electronics, Semiconductor, Spectroscopy

Contaminated Cell Lines are Bad for Your Health | Biofiles

For many decades cell lines have been fundamentally important tools in many fields of biomedical research, including cancer research. Over previous decades many billions of dollars have been spent, a...
Edward Burnett and Liz Penn, European Collection of Cell Culture (ECACC®)
Don Finley, Market Segment Manager, Sigma® Life Science
Biofiles, Vol. 8, No. 16
Keywords: Amplification, Cancer, Cell culture, Polymerase chain reaction

Peer-Reviewed Papers
15

References

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