|Related Categories||A-C, Cell Culture, ECACC Cell Lines More...|
|biological source||colon from human|
|karyotype||Hypertetraploid, modal no. 96|
|shipped in||dry ice|
STR-PCR Data: Amelogenin: X
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsinor Trypsin/EDTA; 5% CO2; 37°C. NB: During routine subculture the cells should always be subcultured before they achieve confluence. Cells may show the appearance of circular vacuoles in the cytoplasm. These may increase in frequency as the culture density increases to confluence. To reduce their frequency, media change confluent cultures after 2-3 days if not subcultured. Cells can clump if not separated into a single cell suspension when split.
Isolated from a primary colonic tumour in a 72-year-old Caucasian male using the explant culture technique. Forms moderately well differentiated adenocarcinomas consistent with colonic primary grade II, in nude mice.
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Caucasian colon adenocarcinoma grade II
without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture
Certificate of Analysis
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For many decades cell lines have been fundamentally important tools in many fields of biomedical research, including cancer research. Over previous decades many billions of dollars have been spent, a...
Edward Burnett and Liz Penn, European Collection of Cell Culture (ECACC®)
Don Finley, Market Segment Manager, Sigma® Life Science
Biofiles, Vol. 8, No. 16
Keywords: Amplification, Cancer, Cell culture, Polymerase chain reaction
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|M7145||MEM Non-essential Amino Acid Solution (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture|
Minimum Essential Medium Eagle, With Earle′s salts and sodium bicarbonate, without
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