Properties
| Related Categories | Cell Culture, ECACC Cell Lines, J-N More... |
| biological source | breast from human |
| growth mode | Adherent |
| karyotype | 2n = 46, hypertriploid to hypotetraploid |
| morphology | Epithelial-like |
| receptors | Reported to express the oestrogen and progesterone receptor |
| shipped in | dry ice |
| storage temp. | −196°C |
Description
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Application
Tumourigenicity and virus studies: Type B and C viruses
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids NEAA + 10% Foetal Bovine Serum FBS / FCS.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsinor Trypsin/EDTA; 5% CO2; 37°C.
Cell Line Description
Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen (P2 containment). Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.
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