86012804 Sigma

A549 Cell Line human

human lung carcinoma

Properties

Related Categories A-C, Cell Culture, ECACC Cell Lines More...
biological source   lung from human
growth mode   Adherent
karyotype   Hypotriploid
morphology   Epithelial
products   Lecithin, High amounts of de-saturated fatty acids.
shipped in   dry ice
storage temp.   −196°C

Description

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Application

Enzymatic studies

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14

Culture Medium

Ham′s F12K or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsin or tTrypsin/EDTA; 5% CO2; 37°C.

Cell Line Description

Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.

Price and Availability


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