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86012804 Sigma

A549 Cell Line human

human lung carcinoma

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Properties

Related Categories A-C, Cell Culture, ECACC Cell Lines More...
biological source   lung from human
growth mode   Adherent
karyotype   Hypotriploid
morphology   Epithelial
products   Lecithin, High amounts of de-saturated fatty acids.
receptors   Not specified
application(s)   cell culture: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Frequently Asked Questions

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Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

Enzymatic studies

Cell Line Origin

Human Caucasian lung carcinoma

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14

Culture Medium

Ham′s F12K or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsin or tTrypsin/EDTA; 5% CO2; 37°C.

Cell Line Description

Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.

Price and Availability

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With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Required for Optimal Growth

With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Fetal Bovine Serum

USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

<SC>L</SC>-Glutamine solution

200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture

Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Peer-Reviewed Papers

References

Set your institution to view full text papers.

Comparison of methods to assess cyclic AMP turnover under conditions of agonist stimulation in 1321N1 astrocytoma cells. Morgan AJ, Murray KJ and Murray RA Biochem. Soc. Trans. 20(2), 136S, (1992)

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In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. Giard DJ, et al. J. Natl. Cancer Inst. 51(5), 1417-23, (1973)

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Antibodies to the alpha q subfamily of guanine nucleotide-binding regulatory protein alpha subunits attenuate activation of phosphatidylinositol 4,5-bisphosphate hydrolysis by hormones. Gutowski S, et al. J. Biol. Chem. 266(30), 20519-24, (1991)

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Calcium mobilization and its desensitization induced by endothelins and sarafotoxin in human astrocytoma cells (1321N1): comparison of histamine-induced calcium mobilization. Yanai K, et al. Naunyn Schmiedebergs Arch. Pharmacol. 346(1), 51-6, (1992)

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Long-term outcome of treatment for Ann Arbor stage 1 Hodgkin's disease: patterns of failure, late toxicity and second malignancies. Vlachaki MT, et al. Int. J. Radiat. Oncol. Biol. Phys. 39(3), 609-16, (1997)

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Protein kinase C activators sensitize cyclic AMP accumulation by intact 1321N1 human astrocytoma cells. Johnson RA and Toews ML Mol. Pharmacol. 37(2), 296-303, (1990)

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Rapid protein kinase C-dependent activation of phospholipase D leads to delayed 1,2-diglyceride accumulation. Martinson EA, Trilivas I and Trilivas JH J. Biol. Chem. 265(36), 22282-7, (1990)

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c-fos and c-jun are induced by muscarinic receptor activation of protein kinase C but are differentially regulated by intracellular calcium. Trejo J and Brown JH J. Biol. Chem. 266(12), 7876-82, (1991)

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Biphasic increase in c-jun mRNA is required for induction of AP-1-mediated gene transcription: differential effects of muscarinic and thrombin receptor activation. Trejo J, et al. Mol. Cell. Biol. 12(10), 4742-50, (1992)

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Dissociation of protein kinase C redistribution from the phosphorylation of its substrates. Trilivas I, McDonough PM and McDonough JH J. Biol. Chem. 266(13), 8431-8, (1991)

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A requirement for Ras protein function in thrombin-stimulated mitogenesis in astrocytoma cells. LaMorte VJ, et al. J. Biol. Chem. 268(26), 19411-5, (1993)

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Lysophosphatidic acid mimics serum-induced sensitization of cyclic AMP accumulation. Kreps DM, et al. FASEB J. 7(14), 1376-80, (1993)

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Mastoparan inhibits phosphoinositide hydrolysis via pertussis toxin-insensitive [corrected] G-protein in human astrocytoma cells. Nakahata N, et al. FEBS Lett. 260(1), 91-4, (1990)

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Activation of protein kinase C inhibits internalization and downregulation of muscarinic receptors in 1321N1 human astrocytoma cells. Hoover RK and Toews ML J. Pharmacol. Exp. Ther. 253(1), 185-91, (1990)

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Serum-induced sensitization of cyclic AMP accumulation in 1321N1 human astrocytoma cells. Johnson RA, et al. Mol. Pharmacol. 39(3), 399-406, (1991)

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The role of the 2- and 3-hydroxyl groups of 1D-myo-inositol 1,4,5-trisphosphate in the mobilisation of calcium from permeabilised human 1321N1 astrocytoma cells. Wilcox RA, et al. Carbohydr. Res. 234, 237-46, (1992)

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Differential effects of antimycin A on endocytosis and exocytosis of transferrin also are observed for internalization and externalization of beta-adrenergic receptors. Liao JF and Perkins JP Mol. Pharmacol. 44(2), 364-70, (1993)

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The characteristics, capacity and receptor regulation of inositol uptake in 1321N1 astrocytoma cells. Batty IH, et al. Biochem. J. 294 ( Pt 1), 49-55, (1993)

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Adenosine inhibits histamine-induced phosphoinositide hydrolysis mediated via pertussis toxin-sensitive G protein in human astrocytoma cells. Nakahata N, et al. J. Neurochem. 57(3), 963-9, (1991)

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ONO NT-126 is a potent and selective thromboxane A2 antagonist in human astrocytoma cells. Nakahata N, et al. Eur. J. Pharmacol. 184(2-3), 233-8, (1990)

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PGJ2 and delta 12PGJ2 inhibit cell growth accompanied with inhibition of phosphoinositide turnover in human astrocytoma cells. Nakahata N, Abe MT and Abe H Prostaglandins 40(4), 405-16, (1990)

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The presence of thromboxane A2 receptors in cultured astrocytes from rabbit brain. Nakahata N, et al. Brain Res. 583(1-2), 100-4, (1992)

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Trifluoperazine inhibits phosphoinositide hydrolysis as a histamine H1-receptor antagonist. Nakahata N, et al. Eur. J. Pharmacol. 208(3), 265-8, (1991)

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Bicarbonate induces sensitization of cyclic AMP accumulation by intact 1321N1 human astrocytoma cells. Toews ML, Arneson-Rotert LJ and Arneson-Rotert SA J. Pharmacol. Exp. Ther. 264(3), 1211-7, (1993)

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[Signal transduction of prostaglandin E2 and thromboxane A2]. Nakahata N Nippon. Yakurigaku Zasshi. 99(1), 1-11, (1992)

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Muscarinic receptor regulation of protein kinase C distribution and phosphatidylcholine hydrolysis. Brown JH, Trilivas I and Trilivas EA Symp. Soc. Exp. Biol. 44, 147-56, (1990)

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