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86012804 Sigma

A549 Cell Line human

human lung carcinoma

Properties

Related Categories A-C, Cell Culture, ECACC Cell Lines More...
biological source   lung from human
growth mode   Adherent
karyotype   Hypotriploid
morphology   Epithelial
products   Lecithin, High amounts of de-saturated fatty acids.
receptors   Not specified
application(s)   cell culture: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Frequently Asked Questions

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Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

Enzymatic studies

Cell Line Origin

Human Caucasian lung carcinoma

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14

Culture Medium

Ham′s F12K or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsin or tTrypsin/EDTA; 5% CO2; 37°C.

Cell Line Description

Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.

Price and Availability

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Required for Optimal Growth

With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Fetal Bovine Serum

USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

<SC>L</SC>-Glutamine solution

200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture

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