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86030307 Sigma

AFRC MAC 18

Reactivity: Anti-Glycoprotein of Chlamydomonas reinhardii

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   from Rat x Rat Hybridoma
ImmunoDon   AO rat spleen
Myeloma   Y3Ag1.2.3
Reactivity   Anti-Glycoprotein of Chlamydomonas reinhardii
application(s)   cell culture | mammalian: suitable
isotype   IgM
shipped in   dry ice
storage temp.   −196°C

Description

Immunogen

2BII, the major glycoprotein fraction of the cell wall of Chlamydomonas reinhardii

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cell Line Origin

Rat Y3Ag1.2.3 x Rat AO spleen

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Cell Line Description

The antibody recognises glycoprotein 2BII. Binding is lost after acid hydrolysis or de-glycosylation of 2BII. The antibody also cross-reacts with cell wall of Lobomonas piriformis. AFRC MAC 18 antibody shows similar fine specificity and properties to AFRC MAC 19 and 20.

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Cultivate Consistency

Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Related Products

recommended

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Description

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D6546 Dulbecco’s Modified Eagle’s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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