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86030310 Sigma

AFRC MAC 57

Reactivity: Anti-Lipopolysaccharide

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   from Rat x Rat Hybridoma
ImmunoDon   LOU/Iap rat spleen
Myeloma   Y3Ag1.2.3
Reactivity   Anti-Lipopolysaccharide
application(s)   cell culture | mammalian: suitable
isotype   IgM
shipped in   dry ice
storage temp.   −196°C

Description

Immunogen

Sonicated bacteroid material from Rhizobium leguminosarum strain 3841 and strain B155

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cell Line Origin

Rat Y3Ag1.2.3 x Rat LOU/Iap spleen

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Cell Line Description

The antibody is directed against lipopolysaccharide components of the bacterial cell wall and also with lipopolysaccharide from free-living cultures of Rhizobium strains 3841 and B155.

Price and Availability


Cultivate Consistency

Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Related Products

recommended

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Description

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D6546 Dulbecco’s Modified Eagle’s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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