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86030313 Sigma

AFRC MAC 65

Reactivity: Anti-Peribacteroid membrane from Pisum sativum

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   from Rat x Rat Hybridoma
ImmunoDon   Lou/Iap rat spleen
Myeloma   Y3Ag1.2.3
Reactivity   Anti-Peribacteroid membrane from Pisum sativum
application(s)   cell culture | mammalian: suitable
isotype   IgM
shipped in   dry ice
storage temp.   −196°C

Description

Immunogen

Peribacteroid material followed by peribacteroid material depleted of bacterial lipopolysaccharide

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cell Line Origin

Rat Y3Ag1.2.3 x Rat LOU/Iap spleen

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Cell Line Description

The antibody directed against peribacteroid membrane from Rhizobium-induced root nodules of Pisum sativum. The antigen is also found in plasma membranes and Golgi bodies, but not in endoplasmic reticulum ER. The antibody also reacts with peribacteroid membranes from nodules of Vicia and lupin.

Price and Availability


Cultivate Consistency

Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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D6546 Dulbecco’s Modified Eagle’s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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