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86100903 Sigma

OX-34

Reactivity: Anti-CD2, rat

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   from Mouse x Mouse Hybridoma
ImmunoDon   BALB/c mouse spleen
Myeloma   NS0/1
Reactivity   Anti-CD2, rat
application(s)   cell culture | mammalian: suitable
isotype   IgG2a
shipped in   dry ice
storage temp.   −196°C

Description

Immunogen

T blasts prepared in mixed lymphocyte reactions with purified rat T helper cells against irradiated spleen

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cell Line Origin

Mouse NS0/U x Mouse BALB/c spleen

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO2; 37°C

Cell Line Description

The antibody binds to all peripheral T cells and most thymocytes. It does not label peritoneal macrophages or B cells, but does label some spleen cells. The thymocyte molecule detected by the OX-34 antibody has a molecular weight of 50-54kDa.

Price and Availability


Cultivate Consistency

Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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Description

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R0883 RPMI-1640 Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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