EMAIL THIS PAGE TO A FRIEND
86100910 Sigma

OX-45

Reactivity: Anti-BCM1, rat

Purchase

Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   from Mouse x Mouse Hybridoma
ImmunoDon   BALB/c mouse spleen
Myeloma   NS0/1
Reactivity   Anti-BCM1, rat
application(s)   cell culture | mammalian: suitable
isotype   IgG1
shipped in   dry ice
storage temp.   −196°C

Description

Immunogen

Rat T blasts prepared by stimulating purified T helper cells with allogeneic irradiated rat spleen cells

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cell Line Origin

Mouse NS0/1 x Mouse BALB/c spleen

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO2; 37°C.

Cell Line Description

The antibody recognises a 45kDa glycoprotein expressed on all haemopoietic and endothelial cells. The antigen is identical to mouse BCM1 antigen. The antibody inhibits allogeneic mixed lymphocyte reactions using lymph node cells as responders and spleen cells as stimulators.

Price and Availability


Cultivate Consistency

Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

recommended

Product #

Description

Add to Cart

R0883 RPMI-1640 Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?