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86112690 Sigma

AFRC MAC 74

Reactivity: Anti-Nitrate Reductase

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   from Rat x Rat Hybridoma
ImmunoDon   F344 rat spleen
Myeloma   Y3Ag1.2.3
Reactivity   Anti-Nitrate Reductase
application(s)   cell culture | mammalian: suitable
isotype   IgG2a
shipped in   dry ice
storage temp.   −196°C

Description

Immunogen

Nitrate reductase purified from the leaves of 6-8 week old spinach plants - Spinacea oleraceal

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cell Line Origin

Rat Y3Ag1.2.3 x Rat F344 spleen

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Cell Line Description

The antibody is specific for NADH nitrite reductase and inhibits reduction of nitrate to nitrate using NADH electron donor. It also inhibits the reduction of nitrate using flavin nucleotides (FMNH2 or FADH2) or methyl viologen. The antibody is directed against NADH-NR if mono and dicotyledons.

Price and Availability


Cultivate Consistency

Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Related Products

recommended

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Description

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D6546 Dulbecco’s Modified Eagle’s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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