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86112691 Sigma

AFRC MAC 79

Reactivity: Anti-Nitrate Reductase

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   from Rat x Rat Hybridoma
ImmunoDon   F344 rat spleen
Myeloma   YB2/3.0 Ag20
Reactivity   Anti-Nitrate Reductase
application(s)   cell culture | mammalian: suitable
isotype   IgG
shipped in   dry ice
storage temp.   −196°C

Description

Immunogen

Nitrate reductase purified from the leaves of 6-8 week old spinach plants - Spinacea deracea

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

Affinity purification of nitrate reductase

Cell Line Origin

Rat YB2/3.0 Ag20 x Rat F344 spleen

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse feeder layers upon resuscitation

Cell Line Description

The antibody is specific for NADH-nitrate reductase of spinach and marrow. It inhibits the reduction of nitrate to nitrite using NADH as elector donor and also inhibits dehydrogenase activity of NADH - Cytochrome c reductase.

Price and Availability


Cultivate Consistency

Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Related Products

recommended

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Description

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D6546 Dulbecco’s Modified Eagle’s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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