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87080702 Sigma

JW393/A

Reactivity: Anti-NP (4-hydroxy-3 nitrophenacetyl)

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   from Mouse + Human Transfectoma
ImmunoDon   Not applicable
Myeloma   J558L
Reactivity   Anti-NP (4-hydroxy-3 nitrophenacetyl)
application(s)   cell culture | mammalian: suitable
isotype   IgA2
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cell Line Origin

Mouse J558L + Mouse VH gene + Human C (alpha 2) gene

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Cell Line Description

This human chimeric antibody is directed against the hapten 4-hydroxy-3 nitrophenacetyl. The V lambda of the endogenous light chain of the plasmacytoma line complements the VNP of the transfected heavy chain to yield the specific antibody. Gene marker: gpt.

Price and Availability


Cultivate Consistency

Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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D6546 Dulbecco’s Modified Eagle’s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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