|Related Categories||A-C, Cell Culture, ECACC Cell Lines More...|
|biological source||skin from human|
|karyotype||Modal no. 45|
|shipped in||dry ice|
For complete product information, please see C32
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
C32 belongs to the C32r type cultures with the marker M2r substituting the original ring marker M2R. Tumours can be produced in nude mice after 1-2 months (1,000,000 cells injected).
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|M7145||MEM Non-essential Amino Acid Solution (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture|
Minimum Essential Medium Eagle, With Earle′s salts and sodium bicarbonate, without
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