|Related Categories||A-C, Cell Culture, ECACC Cell Lines More...|
|biological source||skin from human|
|karyotype||Modal no. 45|
|shipped in||dry ice|
For complete product information, please see C32
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
C32 belongs to the C32r type cultures with the marker M2r substituting the original ring marker M2R. Tumours can be produced in nude mice after 1-2 months (1,000,000 cells injected).
Palmitylation of an amino-terminal cysteine motif of protein tyrosine kinases p56lck and p59fyn mediates interaction with glycosyl-phosphatidylinositol-anchored proteins Shenoy-Scaria AM et al Mol. Cell. Biol. 13, 6385-92, (1993)
The use of 5-fluorocytosine and ketoconazole in the culture of the erythrocytic stages of Plasmodium falciparum and some tumor cell lines. Roth EF, McKitrick JC and McKitrick F Experientia 45(5), 478-80, (1989)
Monoclonal antibody OKM5 inhibits the in vitro binding of Plasmodium falciparum-infected erythrocytes to monocytes, endothelial, and C32 melanoma cells. Barnwell JW, Ockenhouse CF and Ockenhouse DM J. Immunol. 135(5), 3494-7, (1985)
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|M7145||MEM Non-essential Amino Acid Solution (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture|
Minimum Essential Medium Eagle, With Earle′s salts and sodium bicarbonate, without
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