|Related Categories||Cell Culture, ECACC Cell Lines, S-Z More...|
|biological source||Ovary from hamster|
|karyotype||2n = 22|
|shipped in||dry ice|
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Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Derived from parent line CHO-K1. Cells were exposed to increasing concentrations of compactin over 8 months. During this exposure the level of 3-hydroxy-3-methylglutary
Appearance of crystalloid endoplasmic reticulum in compactin-resistant Chinese hamster cells with a 500-fold increase in 3-hydroxy-3-methylglutaryl-coenzyme A reductase. Chin DJ, et al. Proc. Natl. Acad. Sci. U. S. A. 79(4), 1185-9, (1982)
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