87100701 Sigma

UT-1

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   Ovary from hamster
growth mode   Adherent
karyotype   2n = 22
morphology   Epithelial
shipped in   dry ice
storage temp.   −196°C

Description

Application

Enzymatic studies

General description

For complete product information, please see UT-1

Culture Medium

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Cell Line Description

Derived from parent line CHO-K1. Cells were exposed to increasing concentrations of compactin over 8 months. During this exposure the level of 3-hydroxy-3-methylglutaryl coenzyme A reductase synthesis increased 500-fold. The enzyme controls cholesterol synthesis.

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