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87128 Sigma

Tetracycline

≥98.0% (NT)

  • CAS Number 60-54-8

  • Empirical Formula (Hill Notation) C22H24N2O8 · xH2O

  • Molecular Weight 444.43 (anhydrous basis)

  •  Beilstein Registry Number 2230417

  •  EC Number 200-481-9

  •  MDL number MFCD00151232

  •  PubChem Substance ID 24888860

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Properties

Related Categories Antibacterial, Antibiotics, Antibiotics A to Z, Antibiotics T-Z, Antibiotics by Application,
InChI Key   OFVLGDICTFRJMM-WESIUVDSSA-N
assay   ≥98.0% (NT)
optical activity   [α]20/D −230±5°, c = 1% in methanol
impurities   4-11% water
mp   172-174 °C (dec.)
solubility   95% ethanol: soluble12.5 mg/mL
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
storage temp.   2-8°C
Gene Information   human ... CYP1A2(1544), TERT(7015)

Description

Biochem/physiol Actions

Mode of Action: Tetracycline passively diffuses through proin channels in the cell membrane, binding to 30S ribosomes and inhibits protein synthesis by preventing access of aminoacyl tRNA to the acceptor site on the mRNA-ribosome complex. It also binds to the bacterial 50S ribosomal subunit, altering the membrane and causing intracellular components to leak from bacterial cells. The inhibitory effects can be reversed by washing, suggesting that it is the reversibly bound antibiotic, and not the irreversibly bound drug, that is responsible for antibacterial action.

Mode of Resistance: The effects are inactivated via a loss of cell wall permeability.

Antimicrobial spectrum: Includes a wide range of antimicrobial activity against gram-positive and gram-negative bacteria.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
QI8750000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Protocols

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

Peer-Reviewed Papers
15

References

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