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87444 Sigma

5-Carboxy-fluorescein diacetate N-succinimidyl ester

for fluorescence, ≥95.0% (HPLC)

Synonym: 5-Carboxy-di-O-acetylfluorescein N-succinimidyl ester



Related Categories Biochemicals and Reagents, Fluorescein and Derivatives, Fluorescence, Fluorescent Labeling of Amines, Fluorescent Labels,
grade   for fluorescence
assay   ≥95.0% (HPLC)
solubility   DMF: soluble
  DMSO: soluble
fluorescence   λex 492 nm; λem 517 nm
storage temp.   −20°C


Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Other Notes

Continuous determination of the intracellular pH in bacteria by fluorometry; labeling human intervertebral disk cells in vitro for fluorescence microscopy

Price and Availability

Laboratory Gloves

Amplified Detection
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Can product 87444, 5-Carboxy-fluorescein diacetate N-succinimidyl ester, be used in flow cytometry?
Yes, product 87444, 5-Carboxy-fluorescein diacetate N-succinimidyl ester, can be used in flow cytometry. The optimal concentration range for cell labeling is 2.5 uM to10 M. The concentration of the cell line will have to be optimized. See the link below for more information. http://www.abbs.info/pdf/37-6/3-379-385-04244.pdf
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