Properties
| Related Categories | A-C, Cell Culture, ECACC Cell Lines More... |
| biological source | pancreas from rat |
| growth mode | Adherent |
| morphology | Epithelial |
| products | Insulin and glucagon |
| shipped in | dry ice |
| storage temp. | −196°C |
Description
Application
Hormone studies
General description
For complete product information, please see CRI-G5
Culture Medium
DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. The cells tend to grow in clumps and ribbons.
Cell Line Description
Established from a NEDH rat transplantable islet cell tumour. The cells secrete insulin and glucagon. This cell line can be used in place of CRI-D11 (Sigma Catalogue number. 88031604).
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