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89040603 Sigma

YTA 3.1.2

Reactivity Anti-L3T4 epitope B, mouse CD4)

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   from Rat x Rat Hybridoma
ImmunoDon   DA rat spleen
Myeloma   Y3Ag1.2.3
Reactivity   Anti-L3T4 epitope B, mouse CD4
application(s)   cell culture | mammalian: suitable
isotype   IgG2b
shipped in   dry ice
storage temp.   −196°C

Description

Application

Not specified

Immunogen

CBA mouse spleen cells, thymocytes

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Cambridge Enterprise Limited, Hauser Forum, 3 Charles Babbage Road, Cambridge CB3 0GT. Originators: Professor H Waldmann and Dr SP Cobbold when at the Department of Pathology, University of Cambridge

Cell Line Origin

Not specified

Culture Medium

Iscoves Modified Dulbeccos Medium (IMDM) + 2 mM glutamine + 5% Fetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA, trypsin or mechanical agitation maybe required to dislodge them from the plastic.

Subculture Routine

Maintain cultures between 3-9 x 105 cells/ml; 5% CO2; 37 °C. Saturation density 9 x 105/ml

Cell Line Description

The antibody is directed against epitope B of the L3T4 antigen. This antigen is the murine equivalent of CD4. Has been used in CD4 subset depletion in vivo and flow cytometry studies. Cambridge Enterprise Reference number AB13:www.enterprise.cam.ac.uk.

Price and Availability


Cultivate Consistency

Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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