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89040604 Sigma

YTS 156.7.7

Reactivity: Anti-Lyt-3 epitope B, mouse

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   from Rat x Rat Hybridoma
ImmunoDon   DA rat spleen
Myeloma   Y3Ag1.2.3
Reactivity   Anti-Lyt-3 epitope B, mouse
application(s)   cell culture | mammalian: suitable
isotype   IgG2b
shipped in   dry ice
storage temp.   −196°C

Description

Immunogen

CBA mouse thymocytes

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Cambridge Enterprise Limited, Hauser Forum, 3 Charles Babbage Road, Cambridge CB3 0GT. Originators: Professor H Waldmann and Dr SP Cobbold when at the Department of Pathology, University of Cambridge

Culture Medium

DMEM + 2 mM glutamine + 0.05 mM 2-mercaptoethanol (2ME) + 20% Fetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.

Cell Line Description

The antibody is directed against the B epitope of the mouse Lyt-3 (CD8) antigen.Has been used in CD8 subset depletion in vivo and flow cytometry studies. Cambridge Enterprise Reference number AB15: www.enterprise.cam.ac.uk.

Price and Availability


Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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