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89040701 Sigma

ED1

Reactivity Anti-macrophage, rat)

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   from Mouse x Mouse Hybridoma
ImmunoDon   BALB/c mouse spleen
Myeloma   Sp2/0
Reactivity   Anti-macrophage, rat
application(s)   cell culture | mammalian: suitable
isotype   IgG1
shipped in   dry ice
storage temp.   −196°C

Description

Application

Not specified

Immunogen

Highly purified recombinant human tumour necrosis factor alpha

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr C D Dijkstra, Cell Biology and Immunology Dept, Vrije University, Amsterdam

Cell Line Origin

Not specified

Culture Medium

RPMI 1640 + 2 mM glutamine + 10% Fetal Bovine Serum (FBS) (heat inactivated)

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2; 37 °C.

Cell Line Description

The antibody recognises a cytoplasmic antigen on monocytes and on most rat macrophage sub-populations. Please note that this hybridoma produces antibody in very low amounts (0.1 μg/ml).

Price and Availability


Cultivate Consistency

Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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