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89062001 Sigma

HFN 7.1

Reactivity: Anti-Fibronectin, human

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   from Mouse x Mouse Hybridoma
ImmunoDon   BALB/c mouse spleen
Myeloma   P3X63Ag8
Reactivity   Anti-Fibronectin, human
application(s)   cell culture | mammalian: suitable
isotype   IgG1
shipped in   dry ice
storage temp.   −196°C

Description

Immunogen

Affinity purified fibronectin

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cell Line Origin

Mouse P3X63Ag8 x Mouse BALB/c spleen

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Cell Line Description

The antibody is directed against human fibronectin and does not cross-react with fibronectin from the 26 other diverse species tested. The antibody will inhibit cell attachment to collagen, but also inhibit fibronectin collagen binding.

Price and Availability


Cultivate Consistency

Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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Description

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D6546 Dulbecco’s Modified Eagle’s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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