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89062101 Sigma

F10-89-4

Reactivity: Anti-CD45 determinant 1, human

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   from Mouse x Mouse Hybridoma
ImmunoDon   BALB/c mouse spleen
Myeloma   NS1/1
Reactivity   Anti-CD45 determinant 1, human
application(s)   cell culture | mammalian: suitable
isotype   IgG2a
shipped in   dry ice
storage temp.   −196°C

Description

Immunogen

Human T lymphocytes

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cell Line Origin

Mouse NS1/1 x Mouse BALB/c spleen

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 2-5x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder cells upon resuscitation

Cell Line Description

The antibody recognises the human leukocyte common antigen which is found on cells from spleen, lymph nodes, 83% bone marrow cells and granulocytes. The antigen recognised by F10-89-4 is a glycoprotein with a molecular weight of 190kDa to 215kDa.

Price and Availability


Cultivate Consistency

Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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Description

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R0883 RPMI-1640 Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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