89072609 Sigma

Mc 39-16

Reactivity: Anti-A31 idiotype, murine



Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   from Mouse x Rat Heterohybridoma
ImmunoDon   LOU rat spleen
Myeloma   NS1
Reactivity   Anti-A31 idiotype, murine
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C



Sheep anti murine IgM-A31 IgM complexes

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.


The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.

Cell Line Origin

Mouse NS1 x Rat LOU spleen

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Cell Line Description

This heterohybridoma secretes a rat monoclonal antibody against the A-31 idiotype of murine B cell lymphoma. Available for use as a heterohybridoma secreting rat IgG2a antibody. However, use in studies of the A31 tumour requires permission from the originators.

Price and Availability

Cultivate Consistency

Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis

Protocols & Articles
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D6546 Dulbecco’s Modified Eagle’s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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