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89111412 Sigma

Y13-259

Reactivity: Anti-p21 Protein, Harvey Murine Sarcoma Virus

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   from Rat x Rat Hybridoma
ImmunoDon   Osborne-Mendel rat spleen
Myeloma   Y3Ag1.2.3
Reactivity   Anti-p21 Protein, Harvey Murine Sarcoma Virus
application(s)   cell culture | mammalian: suitable
isotype   IgG1
shipped in   dry ice
storage temp.   −196°C

Description

Immunogen

Ha-MuSV transformed syngeneic NRK cells

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cell Line Origin

Rat Y3Ag1.2.3 x Rat Osborne-Mendel spleen

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells will semi-attach. Shake cells off or use trypsin to remove cells from flask

Cell Line Description

The antibody reacts to p21 encoded by Ha-MuSV v-ras gene. Cross-reacts to p21 encoded by Kirsten MuSV v-ras gene, p21 encoded by BALB-SV and 29kDa gag-ras fusion protein encoded by Ra-SV. Recognises p21 species in some uninfected rodent, carnivore and primate lines.

Price and Availability


Cultivate Consistency

Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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Description

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D6546 Dulbecco’s Modified Eagle’s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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