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89120558 Sigma

AFRC MAC 257

Reactivity: Anti-Maize membrane auxin binding protein

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   from Mouse x Rat Heterohybridoma
ImmunoDon   F344 rat spleen
Myeloma   NS0/U
Reactivity   Anti-Maize membrane auxin binding protein
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Immunogen

Purified auxin binding protein

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

The isotype of the monoclonal antibody produced by this cell line is rat IgM.

Cell Line Origin

Mouse NS0/U x Rat F344 spleen

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Cell Line Description

The antibody recognises the 22kDa major auxin binding protein polypeptide and. In addition a minor 21kDa species is recognised.

Price and Availability


Cultivate Consistency

Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
Related Products

recommended

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D6546 Dulbecco’s Modified Eagle’s Medium - high glucose, With 4500 mg/L glucose, sodium pyruvate, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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