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90020107 Sigma

WI 38 Cell Line human

caucasian fibroblast-like fetal lung cell

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   Lung, fetal from human
growth mode   Adherent
karyotype   2n = 46, diploid except at high passage number
morphology   Fibroblast-like
products   Collagen
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Analysis Note

Recommended culture medium: EMEM (EBSS) + 2 mM L-Glutamine + 1% Non-essential amino acids (NEAA) + 15% FBS

Biochem/physiol Actions

Finite lifespan. Approx. 50, +/- 10, population doublings. Normal doubling time is 24 hours.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

From normal female embryonic lung (expect collagen production). These cells have a very broad human virus spectra. Particularly useful for the isolation of rhinovirus. Cells from multilatered membrane when incubated for long periods with periodic pH adjustments. Used in the preparation of human virus vaccines.

Subculture: Spit at 70-80% confluency, approx. 1:3 to 1:6, seed at 2-4x104 viable cells/cm2. Trypsinize using 0.25% solution, with or without EDTA, 37 °C and 5% CO2.

Virus studies (rhinoviruses), vaccine production

Cell Line Origin

Human Caucasian foetal lung

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 10
D7S820: 9,11
THO1: 8,9.3
TPOX: 8
vWA: 19,20

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Finite lifespan, 50 plus or minus 10 population doublings expected - doubling time 24h.

Cell Line Description

From normal embryonic lung of female. Have very broad human virus spectra, especially useful for isolation of rhinoviruses. Cells form multilayered membrane when held for long periods at 37°C with periodic pH adjustments. Used in preparation of human virus vaccines.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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Protocols & Articles
Peer-Reviewed Papers
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References

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M7145 MEM Non-essential Amino Acid Solution (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture
M2279 Minimum Essential Medium Eagle, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
G7513 L-Glutamine solution, 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture

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