|Related Categories||Cell Culture, ECACC Cell Lines, S-Z More...|
|biological source||Lung, foetal from human|
|karyotype||2n = 46, diploid except at high passage number|
|shipped in||dry ice|
Recommended culture medium: EMEM (EBSS) + 2 mM
Finite lifespan. Approx. 50, +/- 10, population doublings. Normal doubling time is 24 hours.
From normal female embryonic lung (expect collagen production). These cells have a very broad human virus spectra. Particularly useful for the isolation of rhinovirus. Cells from multilatered membrane when incubated for long periods with periodic pH adjustments. Used in the preparation of human virus vaccines.
Subculture: Spit at 70-80% confluency, approx. 1:3 to 1:6, seed at 2-4x104 viable cells/cm2. Trypsinize using 0.25% solution, with or without EDTA, 37 °C and 5% CO2.
Virus studies (rhinoviruses), vaccine production
STR-PCR Data: Amelogenin: X
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Finite lifespan, 50 plus or minus 10 population doublings expected - doubling time 24h.
From normal embryonic lung of female. Have very broad human virus spectra, especially useful for isolation of rhinoviruses. Cells form multilayered membrane when held for long periods at 37°C with periodic pH adjustments. Used in preparation of human virus vaccines.
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|M7145||MEM Non-essential Amino Acid Solution (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture|
Minimum Essential Medium Eagle, With Earle′s salts and sodium bicarbonate, without
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